PurR purR (-) purB (-) Others (-) DHypoxanthine (CoR), guanine (CoR) and analogues 163-168 TrpR trpR (-) trpLEDCBA (-) Others (-) D L-tryptophan (CoR) 169,170 TyrR tyrR (-) aroF-tyrA (-) mtr (+) Others (-) U ATP + L-tyrosine ‡‡‡ (CoR, but Ind of mtr ) 171-173 *The first 34 systems are ...
The Arabinose Operon Dr. James Kaper jkaper@umaryland. edu •To understand the mechanism of autoregulation of AraC •To understand the DNA looping ... Soissonet al. 1997 Science 276:421-5 Regulation of the araBAD operon
We also found that the expression of araFGH is more sensitive to catabolite repression but not to arabinose concentration than are araE and araBAD .
This segment includes the promoter for rightward araBAD transcription, p&&s, the promoter for leftward araC transcription, PC, and sites responsible for repression.
Figure 1 - Regulation of the araBAD promoter C C O 2 pBAD I 2 I 1 AraC dimer No transcription L-arabinose N N P c N N C C I 1 I 2 pBAD P c CAP Transcription
Fax: ›15106431228. e-mail: keasling! socrates.berkeley.edu 1 Department of Chemical Engineering, University of California, Berkeley, CA 94720-1462, USA 2 Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA Genes placed under the control of the arabinose-inducible araBAD promoter ...
The DE( araBAD ) 567 and DE( rhaBAD ) 568 mutations correspond to the araBAD AH33 and rhaBAD LD78 alleles (14), respectively. The adjacent rrnB3 lacZ4787 mutations were previ-ouslycalled rrnB T14 lacZ WJ16 (14).
Specifically, without arabinose, it binds and prevents RNAP access to the araBAD promoter—thus serving as a negative regulator of araBAD transcription.
• To induce expression from the araBAD promoter, use L-arabinose (Lee, 1980; Lee et al., 1987). To modulate expression, simply vary the concentration of L-arabinose added.
coli K-12] EMG2 rpoS (Am) rph-1 W1485 F + λ-rpoS (Am) rph-1 MG1655 F-λ-rph-1 BD792 F-λ-rpoS (Am) rph-1 BW25113 rrnB3 Δ lacZ4787 hsdR514 Δ ( araBAD ) 567 Δ ( rhaBAD ) 568 rph - 1 Step 1 Step 2 13 steps Design for construction of in-frame, single gene deletion mutants To isolate a set of E .