Soil samplescollected from NagNadi (River) used for decolourisation and degradation of DirectViolet51 and Tatrazinedyes, on further screening of decolorizing bacteria transferred over HICROM agar containing chromogenic mixture used for differentiation of organism found to be Proteus Sp., Pseudomonas sp. and ...
Then, it was filtrated with 0.45 µm membrane filter and injected into HPLC equipped with Spherisorb 5 µm NH 2 column (250 × 4.6 mm; Waters, Ireland) ; Waters 6000 A pump (Waters, Hicrom Ltd. Uk) ; Refractive Index detector (Waters 2414) .
HPLC apparatus was equipped with Waters 6000A pump (Waters, Hicrom Ltd. Uk); ultraviolet detector (Unicam Analytical Systems, Cambridge, UK) and µBondapak C18 column (Waters, Hicrom) using acetonitrile (12.00 %; pH 4.98) as the mobile phase.
For confirmation of S. aureus strains Hicrom Aureus agar (M1468, Hi Media) was used and following biochemical tests were provided. Those results were confirmed by HiStaph TM-identification kit (Hi Media).
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