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Lipofectin

Lipofectin® Reagent

Lipofectin ® Reagent Part no. 18292.lipo.pps MAN0001376 Rev. Date 14 July 2011 Cat. nos.: Size: Store at 4C (do not freeze) 18292-011 1 mL 18292-037 4 x 1 mL Description Lipofectin ® Reagent is suitable for transfecting DNA (1,2), RNA (3,4), and oligonucleotides (5) into mammalian cells ...

Lipofectamine 2000 Transfection Reagent

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Lipofectamine™ 2000

Lipofectamine ™ 2000 Cat. No. 11668-027 Size: 0.75 ml Cat. No. 11668-019 Size: 1.5 ml Contents and Storage Lipofectamine ™ 2000 is supplied in liquid form at a concentration of 1 mg/ml. Store at +4°C.

Sperm-mediated gene transfer in poultry

Trial 1 was achieved to assess the effects of semen dilution (4 µl diluent/1 µl semen), heat incubation (exposure of semen to 37°C for 30 minutes) and the addition of lipofectin and their combinations on sperm characteristics.

Improved Transfection of Eukaryotic Cells Using FuGENE™ 6 ...

Mv1Lu, 2 mg phGFP-S65T + 3 ml FuGENE 6 Reagent or 5 ml Lipofectin; MC3T3 E1, 2 mg phGFP-S65T + 9 ml FuGENE 6 Reagent or 10 ml Lipofectin; ROS 17/2.8, 1 mg phGFP-S65T + 3 ml FuGENE 6 Reagent; or 2 mg phGFP-S65T + 10 ml Lipofectin.

* Present address: Institute of Microbiology and Genetics ...

EXPRESSING HUMAN a-INTERFERON USING LIPOFECTIN MEDIATED TRANSFORMATION WAGDY A. SAWAHEL* Microbial Genetics Department, Genetic Engineering & Biotechnology

LIPOFECTION PROCEDURE- CHO (PF)

Prepare cells: While DNA is incubating with lipofectin reagent, wash cells with 1xPBS 2 times. Add 10 ml 1% FCS medium (with glutamine). Add DNA to cells: Add lipofectin/DNA mixture drop by drop around the plate.

Lipofectamine Reagent Contents and Storage

Lipofectamine Ž . Reagent Contents and Storage: Lipofectamine is supplied in liquid form at a concentration of 2 mg/ml. Store at 4°C. DO NOT FREEZE.

In vivo liposome-mediated transfection of HLA-DR alpha-chain ...

incubated with lipofectin ® in a 1:3-ratio for 10 min. The DNA:lipofectin ®-complex was diluted to 10 cc with physiologic saline and delivered into the left anterior descending artery of 6 farm pigs over 10 min.

Lipofectin Transfection Activity Increased by Protocol ...

C ationic lipid-mediated transfection is a simple procedure for nucleic acid delivery to a wide variety of cell types (1-3). Cationic liposome reagents are provided in water and are diluted into physiologic saline solutions such as cell culture medium at the time of use.