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Protocol for Neutralizing Antibody Assay for HIV-1 in TZM-bl ...

Montefiori Laboratory Duke University Protocol for Neutralizing Antibody Assay for HIV-1 in TZM-bl Cells (August 2010) I. Introduction This assay measures neutralization in TZM-bl cells as a function of a reduction in Tat-induced luciferase (Luc) reporter gene expression after a single round of ...

Jiang, David C. Montefiori, James Binley and Shan Lu Michael ...

Published Ahead of Print 21 May 2008. €€ J. Virol.€2008, 82(15):7369. DOI: 10.1128/JVI.00562-08. Jiang, David C. Montefiori, James Binley and Shan Lu

Preparation and Titration of HIV-1 Env-Pseudoviruses ...

Duke Central Immunology Laboratory Preparation and Titration of HIV-1 Env-Pseudoviruses (Montefiori Lab) July 2008 I. INTRODUCTION The performance of neutralizing antibody assays under properly standardized, optimized and validated conditions requires accurate titration of virus infectivity.

Comprehensive Antibody - Vaccine Immune Monitoring Consortium ...

Reference Laboratory -Duke Rigorous program of assay standardization, validation, SOP development, interactive training and regular competency and proficiency testing in preclinical and clinical laboratories D. Montefiori Kelli Greene Hongmei Gao Barbara Wolak

ERIC HOLLANDER, M.D.

ERIC HOLLANDER, M.D. Joining the Faculty of Psychiatry at the Montefiore Medical Center University Hospital of the Albert Einstein College of Medicine; and Formerly Esther and

Protocol for Measuring Neutralizing Antibodies Against HIV-1 ...

TZM-bl Assay for Neutralizing Antibodies 1 Protocol for Measuring Neutralizing Antibodies Against HIV-1, SIV and SHIV Using a Luciferase Reporter Gene Assay in TZM-BL Cells Duke University, USA Neutnet code: 2 Contact person: David Montefiori, monte@duke.edu INTRODUCTION: This assay measures ...

MONTEFIORE MEDICAL CENTER COMMUNITY SERVICE PLAN 2010-2013

1 Montefiore Medical Center is a not-for-profit integrated health care delivery system located in Bronx County, New York. Montefiore operates an extensive health care system, which includes three hospital divisions: the Henry and Lucy Moses Hospital Campus in the Northwest Bronx; the Jack D ...

Program of External Proficiency Testing for the TZM-bl ...

Program of External Proficiency Testing for the TZM-bl Neutralizing Antibody Assay! Initial rounds of testing" Assess inter-laboratory variation under conditions of relaxed standardization!

Magnitude and Breadth ofa Nonprotective Neutralizing Antibody ...

Rodriguez-Chavez, 6,a AllanDeCamp, 1 Mike Giganti, 1 Phillip W. Berman, 5 Steve G. Self, 1 and David C. Montefiori 7 1 Vaccine Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington; 2 Monogram Biosciences, 3 Global Solutions for Infectious Diseases, South San Francisco, 4 PaxVax ...