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Minireview Antisense Oligonucleotides: Basic Concepts and Mechanisms Nathalie Dias and C. A. Stein 1 Columbia University, New York, New York 10032

Oligonucleotide Yield Resuspension and Storage

at Integrated DNA Technologies from the synthesis of millions of oligonucleotides shows that some sequences will, thus, result in better yields than others.

Antisense Oligonucleotides

Product Profile Custom Oligo Synthesis, antisense oligos, RNA oligos, chimeric oligos, Fluorescent dye labeled oligos, Molecular Beacons, siRNA, phosphonates Locked Nucleic Acids (LNA); 2'-5' linked Oligos Antisense Oligonucleotides For research use only.

Resuspending freeze-dried oligonucleotides

Oligonucleotides (Manfred Binder and David Hibbett 9/18/2003) Resuspending freeze-dried oligonucleotides Oligonucleotides are usually shipped in dry form.

ApplicAtion SolutionS for oligonucleotideS

2 Determination of N-glycosylation sites and site heterogeneity in a monoclonal antibody by electrospray quadrupole ion-mobility time-of-flight

Anneal complementary pairs of oligonucleotides

TECH TIP # 45 Pierce Biotechnology PO Box 117 (815) 968-0747 www.thermo.com/pierce 3747 N. Meridian Road Rockford, lL 61105 USA (815) 968-7316 fax Introduction Many methods can be used to anneal complementary strands of nucleic acids.

Mass Spectrometry Analysis of Oligonucleotide Syntheses

In the case of oligonucleotides, multiple protons are removed resulting in a variety of multiply negatively charged species. A quadrupole mass analyzer acts as a variable mass filter that separates ionized

Generating a Synthetic Genome by Whole Genome Assembly ...

1 Generating a Synthetic Genome by Whole Genome Assembly: phiX174 Bacteriophage from Synthetic Oligonucleotides Hamilton O. Smith, Clyde A. Hutchison III *, Cynthia Pfannkoch, and J. Craig Venter The Institute for Biological Energy Alternatives 1901 Research Blvd., #600 Rockville, MD 20850 ...

Antisense oligonucleotide therapy for neurodegenerative disease

Infusion of oligonucleotides into Rhesus monkeys . Under anesthesia, catheters were surgically implanted in the left lateral ventricles of 5 Rhesus monkeys approximately 3 years of age, after which the animals recovered for 5 days.

Analysis of Oligonucleotides by HPLC - Electrospray ...

Analysis of Oligonucleotides by HPLC - Electrospray Ionization Mass Spectrometry Alex Apffel,*,† John A. Chakel, † Steven Fischer, ‡ Kay Lichtenwalter, † and William S. Hancock † Biomeasurements Group, Hewlett-Packard Laboratories, Palo Alto, California 94304, and California Analytical ...