1 The Pichia System James Cregg, Ph.D., Keck Graduate Institute, Claremont, Calif. Introduction Pichia pastoris is a highly successful system for production of a wide variety of recombinant proteins.
User Manual EasySelect ™ Pichia Expression Kit For Expression of Recombinant Proteins Using pPICZ and pPICZ α in Pichia pastoris Cat. no. K1740-01 Rev. Date 18 June 2010 Manual part no. 25-0172 MAN0000042
Pichia pastoris Expression System, continued Two Alcohol Oxidase Proteins Two genes in Pichia pastoris code for alcohol oxidase- AOX1 and AOX2.
Fermentation Strategies for Recombinant Protein Expression in the Methylotrophic Yeast Pichia pastoris
Two Pathways of Sphingolipid Biosynthesis Are Separated in the Yeast Pichia pastoris Philipp Ternes* ‡1, Tobias Wobbe* §, Marnie Schwarz* ‡2
Int. J. Mol. Sci. 2011 , 12 , 3366-3380; doi:10.3390/ijms12053366 International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Article Expression of an Endo-β-1,4-glucanase Gene from Orpinomyces PC-2 in Pichia pastoris Xin Jin, Nan Meng and Li-ming Xia * Department of ...
Electroporation of P. pastoris Transformation of P. pastoris strain X-33 was achieved by electroporation with Pme I-linearized pPICZ*A-BMP and pPICZ*A (negative control) plasmids.
UBC Basic Techniques in Pichia Fermentation Workshop January 15th to January 19th, 2007 Course Title : Basic Techniques in Pichia Fermentation Workshop including operation, optimization and scale up of the Pichia pastoris expression systems.
Pichia pastoris Protocol for Growth in a Fermentor 1 of 12 High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation By Julia Cino, PhD Introduction Over the last several decades, geneticists have learned how to manipulate DNA to identify, excise, move and ...
UNIT 5.7 Overview of Protein Expression in Pichia pastoris During the early 1970s, the methylotrophic yeast Pichia pastoris was developed as a biological tool to convert methanol—a cheap and readily available carbon source that was being discarded by the oil industry as a waste product—into ...