PKH67 Fluorescent Cell Linker Kits For General Cell Membrane Labeling Catalog Numbers MINI67, MIDI67, and PKH67GL Store at Room Temperature TECHNICAL BULLETIN Product Description The PKH67 Fluorescent Cell Linker Kits useproprietary membrane labeling technology to stably incorporate a green ...
PKH26 Red Fluorescent Cell Linker Kits for General Cell Membrane Labeling Catalog Numbers MINI26 andPKH26GL Store at Room Temperature TECHNICAL BULLETIN Product Description The PKH26 Fluorescent Cell Linker Kits useproprietary membrane labeling technology to stably incorporate a yellow ...
PKH67 labelling with high S phase and no significant pro liferation with PKH67 labelling, The discrepancy between the two methods can be explained by the fact that BrdUrd
PKH67 Staining and Culture Procedures Ficoll-purified PBMCswerestained with PKH67 accord-ingtothe manufacturer'sinstructions (Sigma) and as pre-viouslydescribed (23).
The Alginate-PKH67-labeled MSCscompositewas then maintained in a1:1 mixtureofthe osteogenic medium and incubated under conditions identical to those used for the preparation of the Alginate-MSCscomposite.
Peripheral blood mononuclear cells (PBMCs), tracheobronchial lymph node (TLN) and lateral retropharyngeal lymph node (LLN) cells were labeled with PKH67 green fluorescence dye to measure cell proliferation and surface phenotypes were examined using anti-CD4, anti-CD8 or anti-CD25 monoclonal antibodies.
To evaluate lymphocyte subset proliferative responses, PKH67 assay, a flow cytometric pro-liferationassay was used. The PKH67 prolif-erationassaywas performed according to manufacturer instructions (Sigma Chemical Co.) and as previously described (Waters et al., 2000a).
(90%) with PKH67 was determined by FACS analysis as described pre viously (17). BALB/c mice 7 wk of age were injected i.v. with PKH67 labeled exosomes obtained from TS/A cells (10 mice/group) or unlabeled
Invest. 36:665-385 (2007) Methods -1: •Magnetically isolate NK cells from human PBMC •Label K562 target cells with PKH67 •Co-incubate NK and K562 •Gate on NK cells (FSC vs. SSC) and monitor •Acquisition of target cell membrane (PKH67) •CD107a expression (vesicle membrane protein, transiently ...
and PKH67 (Sigma), according to the manufacturer’s instruction, and washed in PBS . PKH26-labelled exosomes (Red) and PKH67 labelled BT-474 cells (Green) were seeded onto 24-well plates and