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Application of Agarose Gel Electrophoresis to the ...

plasmid were obtained, and the identities of the different plasmids could not be determined with certainty. Partially purified plasmid DNA preparations from these strains were used

ILA Application Note

1 Escherichia coli Plasmid Copy Number Assay INTRODUCTION For the plasmid copy number assay, two pairs of probes are required, one pair for the plasmid determination and one pair for the chromosome determination.

First Detection of the Plasmid-Mediated Class A Carbapenemase ...

bapenemase, and Escherichia coli XL-10 transformants were selected with ampicillin (50 g/ml). Extracts of a transformant having a positive bioassay result revealed transfer of the pI 7.0

Bacterial Transformation and Plasmid Recovery

2 I NTRODUCTION When Frederick Griffith observed the conversion of Pneumococcus colonies from 'rough' to 'smooth', he did not realize that the change was due to the uptake of DNA from the dead 'smooth' cells by the live 'rough' cells.


Replication A. Plasmid DNA must replicate every time host cell divides or it will be lost B. Host cells do not "spit out" plasmid DNA C. Two functions of replication 1.

The Art of Plasmid Purification

Gigapreps • Minipreps • Midipreps • Maxipreps • Mega • Gigapre Gigapreps • Minipreps • Midipreps • Maxipreps • Megapreps • Gig Gigapreps • Minipreps • Midipreps • Maxipreps • Megapr • Gigap Gigapreps • Minipreps • Midipreps • Maxipreps • Megapreps • Gig ...

Isolation of a DNA Plasmid in the Fungus Rhizoctonia solani

Journal of General Microbiology (1984), 130, 2067-2070. Printed in Great Britain 2067 Isolation of a DNA Plasmid in the Fungus Rhizoctonia solani By T. HASHIBA,l* Y. HOMMA,* M. HYAKUMACH13 AND I. MATSUDA' National Institute of Agricultural Sciences, Yatabe, Ibaraki-ken 305, Japan 2Shikoku ...

shRNA Plasmids (29-mer) ApplicATion Guide - HuSH

This plasmid has been validated for transient transfection and for the ability to inhibit targeted gene expression with GFP, luciferase and HER2 oncogene specific hairpin DNA inserts.

What is Plasmid DNA?

Plasmid DNA Plasmid DNA 2 What is Plasmid DNA? Bacterial plasmids are closed circular molecules of double-stranded DNA that range in size from 1 to >200 kb.

DNA Technology - Mapping a plasmid

- 1 - DNA Technology - Mapping a plasmid A first step in working with DNA is mapping the DNA molecule. One way to do this is to use restriction enzymes (restriction endonucleases) that are naturally found in bacteria to cut the DNA molecule into fragments, and then perform a gel electrophoresis ...