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Quantitating

Using spectrophotometer to quantitate DNA and RNA

For quantitating DNA or RNA, readings should be taken at wavelengths of 260 nm and 280 nm. The reading at 260 nm allows calculation of the concentration of nucleic acid in the sample. 1 O.D. at 260 nm for double-stranded DNA = 50 ng/ul of dsDNA 1 O.D. at 260 nm for single-stranded DNA = 20-33 ng/ul of ...

Overview of common fluorescent dyes used in Nucleic Acid ...

Some applications of these dyes include: YOYO-1 for measuring DNase activity, quantitating oligionucleotides and PCR products YO-PRO-1 for quantitating dsDNAin solution (reported sensitivity of 2.5 ng/mL) TOTO-1 for quantitating PCR amplification products PO-PRO-3 for quantitating DNA Additional cyanine ...

Quant-iT™ OliGreen® ssDNA Reagent and Kit

Revised: 20-November-2008 | MP 07582 Quant-iT™ OliGreen® ssDNA Reagent and Kit Catalog nos. O7582, O11492 Introduction Quant-iT™ OliGreen® ssDNA reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution ...

Most Probable Number

Figure 1: Three-tube design for MPN (unincubated). = = = 10-1 = = = 10-2 = = = 10-3 The method assumes a random distribution of micro­ organisms in the sample and an accurate dilution of the sample through the dilution series.

€ Bennink, Andrew McMchael and Jonathan W. Yewdell Hickman ...

Quantitating T Cell Cross-Reactivity for Unrelated Peptide Antigens 1 Jeffrey Ishizuka,* † Kristie Grebe,* Eugene Shenderov,* † Bjoern Peters,

ENVIRONMENTAL LABORATORY APPROVAL PROGRAM CERTIFICATION MANUAL

ENVIRONMENTAL LABORATORY APPROVAL PROGRAM CERTIFICATION MANUAL SUBJECT Polarized-Light Microscope Method for Identifying and Quantitating Asbestos in Non-Friable Organically Bound Bulk Samples DATE 11/22/06 PAGE 1 of 25 ITEM NO. 198.6 1.

assays: protein

A simplified method of quantitating protein using the biuret and phenol reagent. Anal. Biochem. 86 , 193-200. 3. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951).

Hoechst Assay Linearity, Reproducibility and Sensitivity

... Scientific - NanoDrop products Wilmington, Delaware USA Technical support: info@nanodrop.com 302-479-7707 www.nanodrop.com Introduction Hoechst 33258 dye is a fluorescent nucleic acid stain for quantitating ...

Procedures for quantitating chlorinated hydrocarbons

Appendix 7 Standard Operating Procedure for the USGS Reston, Virginia Environmental Organic Geochemistry Laboratory Instrumental Analysis for Chlorinated Hydrocarbons Any use of trade, product, or firm names in this publication is for descriptive purposes only and does not imply endorsement by ...

Dilution Analysis Modeling Lymphocytes in Immune Responses by ...

Quantitating Effector and Regulatory T Lymphocytes in Immune Responses by Limiting Dilution Analysis Modeling 1 Thierry Bonnefoix,* Philippe Bonnefoix,* Pascal Perron,* Jian-Qing Mi,* Wan Fai Ng,