In any case, the already mentioned relationship of MITEs and class II transposons suggest that they are mobilised by enzymes closely related to class II transposases ( Feschotteetal., 2002a ).
C OMMUNICATION The Structure ofa Filamentous Bacteriophage Ying A. Wang 1, Xiong Yu 1, Stacy Overman 2, Masamichi Tsuboi 2 GeorgeJ. Thomas Jr 2 ⁎ and Edward H. Egelman 1 ⁎ 1 Department of Biochemistry and Molecular Genetics , University of Virginia , Box 800733, Charlottesville , VA 22908 ...
ing Myc-tagged piggyBac transposases (ranging from 50 to 300 ng) into HEK 293. PiggyBac transposition activity increases as the amount of piggyBac transposases
The observed excision of one Stowaway by two distinct transposases and the excision of two different Stowaways by the same transposase suggest that cross-mobilization maybe a major mechanism for the amplification of rice MITEs.
Comparative analysis of Leifsonia xyli IS elements with putative transposases of Actinobacteria Figure 4 Comparative analysis of Leifsonia xyli IS elements with putative transposases of Actinobacteria .
However, in order for transposon technology to be commercially viable in these areas, it will be necessary to create "hyperactive" transposases, enzymes that can mobilize transposons with extreme efficiency.
The intrinsic activity of the SB sys-temhasbeen enhanced through a variety of measures includ-ingthecreationof hyperactive SB transposases [9,10] , and by engineering transposons with identical 3 0 and 5 0 inverted repeats (IR) [10] .
In this article, we discuss the opportunities provided by natural DNA-modifying enzymes, such as transposases, recom-binasesandintegrases, to mediate the stable integra-tionofforeign genes into host genomes.
Stowaway families are represented by consensus sequences that transposases, the intron/exon structure of the transpo-sasegene, and a comparison of terminal sequences, rice range in size from 96 to 312 bpwith TIRsof 21 to 94 bp (Figure3; consensus sequences were deposited in MLEscouldbe divided into 25 ...
ory has recently been proposed: Tn21 family transposases could recognize the IS4321 ends, and as a result, when IS4321 inserts into one end of a Tn21 family transposon, it may promote the