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Immunophenotyping peripheral blood cells
For the BL3 Vybrant ® DyeCycle ™ Ruby Stain compensation control, return the FSC threshold setting to "Ignore", and return the scatter voltages to those optimized for cells.
Assay Kits and Reagents for Apoptosis, Viability and ...
Vybrant Apoptosis Assay Kits based on phosphatidylserine exposure. Kit Name Catalog Number Annexin V Conjugate Necrotic Cell Stain Application Notes Vybrant Apoptosis Assay Kit #1 V-13240 Alexa Fluor 488 SYTOX Green Flow cytometryUses only the green (FL1) channel to detect all three cell populations, leaving the rest ...
Tips and Tricks
Simple Protocols • 1.1 Remove the Vybrant® DyeCycle™ Violet stain from the refrigerator and allow it to equilibrate to room temperature. • 1.2 Prepare flow cytometry tubes each containing 1 mL of cell suspension in complete media at a concentration of 1 × 10 6 cells/mL. • 1.3 To each ...
The flow cytometry violet laser resource guide
Vybrant® DyeCycle™ Violet Stain is DNA selective, cell membrane-permeant, and nonfluorescent until bound to double-stranded DNA. Cell cycle analysis based on Vybrant® DyeCycle™ Violet staining results in a clear pattern of distribution: G 0 /G 1 phase (one set of paired chromosomes per cell), S ...
Protocol: Kinetic measurement of apoptosis using CellPlayerTM ...
Dilutions of Vybrant DyeCycle Green stain can be made in either culture medium or PBS prior to addition to the wells 2) Add diluted Vybrant DyeCycle Green stain directly to the well immediately after the final caspase-3/7 scan.
New Molecular Probes Flow Cytometry Products from Invitrogen
Enabling Your Multi-Color Experiments Tools for Cell Cycle Analysis Vybrant® DyeCycle Ruby Stain - NEW ! FxCycle™ Violet Stain- NEW! FxCycle™ Far Red Stain- NEW!
Approaches to Cell Proliferation and Cell Counting.
Invitrogen Proprietary & Confidential Technologies Invitrogen Offers. • Cell Number by Metabolic Indicators-Vybrant Cell Metabolism Assay Kit-LIVE/DEAD Calcein AM Vitality Kits-Luminescent: ATP Determination Kit-Colorimetric: Alamar Blue, MTT • Cell Number by DNA Content-CyQuant™, CyQuant ...
Cell Proliferation and Cell Cycle Analysis using a Multiple ...
Vybrant ® DyeCycle TM Violet Propidium Iodide DNA Stain G1 Phase (pEC50) G2/M Phase (pEC50) n Propidium Iodide 8.19 ± 0.06 8.04 ± 0.06 5 Vybrant ® DyeCycle TM Violet 8.15 ± 0.07 8.00 ± 0.05 3 Live cells were stained with Vybrant® DyeCycle TM Violet and analysed on an Acumen e X3 microplate cytometer using 405 nm ...
Fluorescence Tools for Analysis of Apoptosis
12 Vybrant ® Apoptosis Kit #10 4 hours Necrotic Annexin V-APC SYTOX Green Dye C 12 resazurin C 12-resazurin resorufin Reductase 488 nm & 633 nm Excitation
CellPlayer™ 96-Well Kinetic Caspase-3/7 Apoptosis Assay
For DNA object count at the assay end point, all DNA containing objects were labeled using the DNA-binding dye Vybrant DyeCycle Green (Life Technologies).
