... JC-1/DiOC2(3) Fixable Red Dead Cell Stain 7-AAD SNARF ® (high pH) isomers SYTOX ® AADvanced ™ Dead Cell Stain DyeCycle ™ Ruby Blue/Red 690/50 665-715 Qdot ® 705 PerCP TRI-COLOR ® (TC) PE-Cy ® 5 PerCP-Cy ® 5.5 PE-Cy ® 5.5 Vybrant ® Dye Cycle ™ Ruby Blue/Red 780/60 750-810 PE-Alexa Fluor ® 750 Qdot ® 800 PE-Cy ® 7 Vybrant ® Dye Cycle ...
After 18-24hr, the Vybrant™ Cytotoxicity Assay (Molecular Probes) was used to measure the amount of glucose 6-phosphate dehydrogenase released from damaged cells.
Vybrant DiO or FAST DiO and finally the overlay image of the nuclear and membrane stains. Figs. 4A–E shows a 2-cell embryo simultaneously labeled with CTβ/AF594 (Fig. 4C)
HAF and HBC were labelled with Vybrant TM DiD and Vybrant TM DiI, respectively (Molecular Probes, Luzern, Switzerland) following the procedure described by Kaiser and Bruinink (Kaiser and Bruinink, 2004).
imidyl ester (CFDA-SE) was performed using the Vybrant CFDA-SE cell tracer kit from Molecular Probes. Priming HY-specific CD8 T cells Splenocytesusedforimmunizationwereisolatedfrommalemice.CD11c
After 15min, the chitosan microstructures with cells were gently washed withPBSand transferred to fresh 6-well plates. 24 hafter transfer, NIH-3T3 cells weretrypsinized, stained with Vybrant Dilandplatedfor co-culture atadensityof 1*10 6 cells/well.
presence and absence of BCG by using the Vybrant™ Cytotoxicity Assay and CellTiter96® AQueous One Solution Cell Proliferation Assay, respectively.
Apoptosis induction was determined using the Vybrant apoptosis assay kit. This assay utilizes two dyes, Propidium Iodide (PI) and YO-PRO -1.
Fluorescence intensity was measured in the wells using the appropriate excitation and emission filters: Calcein excitation/emission: Fluorescein 485 nm/Fluorescein 530nm; EthD-1 excitation/emission: Rhodamine B/Alamar 535-05/Europium 620nm Vybrant TM Cytotoxicity Assay The Vybrant Cytotoxicity kit ...
| Proprietary and Confidential. 4 Approaches to Cell Number Determination • Cell Number by Metabolic Indicators-Vybrant Cell Metabolism Assay Kit-LIVE/DEAD Calcein AM Vitality Kits-Luminescent: ATP Determination Kit-Colorimetric: Alamar Blue, MTT • Cell Number by DNA Content-Cy Quant™, CyQuant™NF ...